sarah.morrison said:Concentration choice is a precision decision, not a preference.
I am going to disagree with reconstituting low as a general rule. More diluent, more punctures, more in-use days at room temperature, and the stability trade-off is real. Precision is not the only variable being optimised.
TrialTracker_MD said:I reconstituted a 10mg vial with 2ml of bacteriostatic water and I want someone to check my unit arithmetic before I draw anything, because I keep…
Peptide calculator app recommendation for dosing arithmetic: I use PeptideCalc to calculate reconstitution volumes and injection doses. Eliminates math errors completely.
Input: vial size (mg), desired concentration (mg/mL), prescribed dose (mg)
Output: BAC water volume (mL), injection volume (units on insulin syringe)
A math error with injectable medication can mean underdosing or overdosing. Use a calculator every time until the math becomes second nature.
MikeFit_NJ said:I am going to disagree with reconstituting low as a general rule.
Reconstitution tip for anyone mixing dosing arithmetic from lyophilized powder: use bacteriostatic water, NOT sterile water. The benzyl alcohol in BAC water preserves multi-dose vials for up to 28 days.
Inject the water slowly down the side of the vial. NEVER shake — gentle swirling only until fully dissolved. Should be crystal clear with no particles.
Sigma-Aldrich — Research-Grade Standards
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Shop Reference StandardsOne thing that is still open after ben_calgary’s answer:
How much material I am losing to dead space, and whether that explains why a 10mg vial gives me nine usable draws rather than ten?
OP back with an update, since a thread like this is useless without one.
Resolved. I wrote mg, ml and mg/ml on the vial in marker and did the division in that order, and the two of us now get the same number every time.