Dr.BariatricHTX said:They are not contradictory, they measure different things, and this is the single most common misreading of a certificate.
I am more sceptical of certificates as a category than this thread is. The document tells you about a sample somebody chose to send, at a moment in time, and nothing about the vial in your hand. Independent purchase-and-test is a different quality of evidence and everything else is inference.
Dr.RaviCardio said:Two reports on the same product from two laboratories, four percentage points apart, and no method stated on either.
My HPLC chromatogram analysis for certificates of analysis — I sent my batch to Janoshik and here's what the results showed:
Main peak retention time: 16.3 minutes (consistent with semaglutide reference standard)
Peak purity: 98.5%
Related substances: 0.7% (single impurity at 10.2 minutes — likely a synthesis intermediate)
Mass spec: m/z 4113.5 (matches expected molecular weight)
This is a clean result. The mass spec confirmation is particularly important — it proves the molecule is actually semaglutide and not a substitution.
SarahChen_PharmD said:I am more sceptical of certificates as a category than this thread is.
Third-party testing services for certificates of analysis besides Janoshik:
- Janoshik: Gold standard for peptide community, ~$153, 10-14 day turnaround
- Valitech: US-based, ISO 17025 accredited, ~$203
- Eurofins: Full pharmaceutical testing suite, more expensive but comprehensive
- ChemTox: Basic identity and purity testing, ~$113
Janoshik is the community standard but any ISO-accredited analytical lab can verify peptide identity and purity. The key is independence — don't rely solely on the vendor's own testing.
PeptideDetective — Independent Peptide Analytics
Community-driven peptide testing and vendor rating platform. Transparent results. Unbiased analysis. Trusted by thousands.
View ResultsA narrower follow-up, since the general answer is now clear:
Whether a purity figure and a content figure can disagree without one of them being wrong?
Reporting back.
Purity and content being different measurements was the piece I was missing. The vial was overfilled and pure, which is a very different problem from the one I thought I had.